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Pcdna V His A B And C

This post categorized under Vector and posted on November 17th, 2019.
PcDNA Vector Map 3: Pcdna V His A B And C

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All pcDNA vectors contain a strong promoter for high-level expression in mammalian cells a choice of selection marker for generating stable cell lines and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in thre pcDNA6V5-His A B and C pcDNA6V5-His A B and C are 5.1 kb vectors derived from pcDNA3.1() and designed for high-level expression selection on blasticidin purification and detection of recombinant proteins in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. pcDNA4V5-His. Consult the multiple cloning sites described on pages 35 to determine which vector (A B or C) should be used to clone your gene in-frame with the C-terminal V5 epitope and the polyhistidine tag. Ligate your insert into the appropriate vector and transform into E. coli.

iv Contents and Storage Contents 20 g each of pcDNA3.1V5-His A B and C are supplied at 0.5 gl in 10 mM Tris-HCl 1 mM EDTA pH 8.0 in a total volume of 40 l. In addition pcDNA4TOmyc-His offers a c-myc epitope for rapid detection of the recombinant protein with an Anti-myc Antibody and a polyhistidine (6xHis) sequence for simple purification of the recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody. user guide For Research Use Only. Not for human or animal therapeutic or diagnostic use. pcDNA4His A B and C Catalog number V862-20 Revision date 9 July 2012

iv Kit Contents and Storage Find and Storage pcDNA3.1myc-His vectors are shipped at room temperature. Upon receipt Gallery vectors at -20C. R V Bst X I Not I Xho I Bst E II Xba I Apa I Sac II Sfu I Pme I V5 epitope Age I 6xHis Term There is a unique Sac II site between the Apa I site and the Sfu I site in version B only. There is a unique BstE II site but no Xba I or Apa I sites in version C. T7 pcDNA4V5-His A B C 5 kb P C M V S V 4 0 o r i A m p i c i l l i n BGHp The pcDNA4HisMax A B & C vectors 20 g each are supercoiled and provided lyophilized. 20 g of pcDNA4HisMaxlacZ control plasmid is also provided. Gallery vectors at -20C. The pcDNA4HisMax TOPOTA Expression Kit contains two boxes.


PcDNA Vector Map 3 Gallery