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Pcdna Gw Emgfp Mir Control P

This post categorized under Vector and posted on November 17th, 2019.
PcDNA Vector Map 3: Pcdna Gw Emgfp Mir Control P

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The BLOCK-iT Pol II miR RNAi Expression Vector Kit with EmGFP combines the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript. The pcDNA6.2-GW P C M V TKpA S V 4 0 o r i f1 o ri B l a s t i c i d i n E M 7 SV40 pA p U C ori pcDNA6.2-GW EmGFP-miR-neg control plasmid 5759 bp Comments for CMV promoter attB1 site EmGFP EmGFP forward sequencing primer site Each pcDNA6.2-TOPO Expression Vector Kit contains two boxes. The TOPO box contains linearized and topoisomerase I-activated pcDNA6.2-TOPO vector control template primers for sequencing PCR reaction components and an expression control plasmid. The TOPO box should be Galleryd at -20C.

The pcDNA 6.2-GWmiR and pcDNA 6.2-GWEmGFP-miR vectors (Figure 1) included in the BLOCK-iT Pol II miR RNAi Expression Vector Kits and the BLOCK-iT graphictiviral Pol II miR RNAi Expression Systems are designed to express artificial miRNAs which are engineered to have 100% graphiclogy to your target sequence and will result in target cleavage. pcDNA6.2-GWmiR or pcDNA6.2-GWEmGFP-miR 1. Set up the following ligation reaction. 5X Ligation Buffer 4 l pcDNA6.2-GW EmGFP-miR (5 ngl) linearized 2 l ds oligo (10 nM 15000 dilution) 4 l DNaseRNase-Get water 9 l T4 DNA Ligase (1 Ul) 1 l Total volume 20 l 2. Mix reaction well and incubate for 5 minutes at BLOCK-iT HiPerform graphictiviral Pol II miR RNAi Expression System with EmGFP carries the WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepagraphicis virus and cPPT (central polypurine tract) from the HIV-1 integrase gene. The pgraphicti6.4R4R2V5-DEST MultiSite Gateway vector is designed to achieve elevated EmGFP

Pcdna6 2 Gw Emgfp Mir Vector supplied by Thermo Fisher used in various techniques. Bioz Stars score 99100 based on 6 PubMed citations. ZERO BIAS - scores article reviews protocol conditions and more Sequencing the expression construct is not required as transfer of the miR RNAi cgraphicette from pcDNA6.2-GWEmGFP-miR into the pgraphicti6.4R4R2V5-DEST MultiSite Gateway vector preserves the orientation of the cgraphicette. However if you wish to sequence your pgraphicti6.4 expression construct we recommend using the following primers. The Vivid Colors Fluorescent Protein Gateway Destination Vectors allow you to quickly and easily fuse a protein of interest to the widely used and well-characterized fluorescent proteins from the jellyfish Aequorea victoria (12) using the pcDNA 6.2 Gateway mammalian expression vector. These powerfu


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