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Plko Vector Cloning

This post categorized under Vector and posted on August 6th, 2019.

Plasmid pLKO.1 - TRC cloning vector from Dr. David Roots lab contains the insert stuffer and is published in Cell. 2006 Mar 24. 124(6)1283-98. This plasmid is available through Addgene. This plasmid is available through Addgene.A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium. The pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has built a library of shRNAs directed against 15000 human and 15000 mouse genes. Addgene is working with the TRC to make this shRNA cloning vector available to the scientific community.pLKO.1 Stuffer-containing graphictiviral vector for expression of shRNA. This plasmid is the official TRC cloning vector.

Addgene inc plko 1 trc cloning vector Plko 1 Trc Cloning Vector supplied by Addgene inc used in various techniques. Bioz Stars score 99100 based on 39 PubMed citations.Vector Name pLKO.1 Alias pLKO_TRC001 Pol II Cgraphicette 1 PGK-PuroR Pol II Cgraphicette 2 na Pol III Promoter consgraphicutive hU6 Pol III Insert 1.9 KB StufferWhich packaging systems are compatible with pLKO.1 shRNA vectors The MISSION TRC shRNAs are cloned into the pLKO.1 transfer vector that is compatible with standard 2 plasmid (packaging vector with rev gene and envelope vector) or 3 plasmid (packaging vector without rev gene envelope vector and rev expression vector) packaging systems.

TRC2 Vector TRC2-pLKO-puro Vector and Use The TRC2-pLKO-puro vector also allows for transient or stable transfection of the shRNA as well as production of graphictiviral particles.

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